Mass spectrometry-based protein-protein interaction techniques and their applications in studies of DNA damage repair     

Zhen CHEN  Junjie CHEN 《Journal of Zhejiang University. Science. B》2021,22(1):1-20

Proteins are major functional units that are tightly connected to form complex and dynamic networks.These networks enable cells and organisms to operate properly and respond efficiently to environmental cues.Over the past decades,many biochemical methods have been developed to search for protein-binding partners in order to understand how protein networks are constructed and connected.At the same time,rapid development in proteomics and mass spectrometry(MS)techniques makes it possible to identify interacting proteins and build comprehensive protein-protein interaction networks.The resulting interactomes and networks have proven informative in the investigation of biological functions,such as in the field of DNA damage repair.In recent years,a number of proteins involved in DNA damage response and DNA repair pathways have been uncovered with MS-based protein-protein interaction studies.As the technologies for enriching associated proteins and MS become more sophisticated,the studies of protein-protein interactions are entering a new era.In this review,we summarize the strategies and recent developments for exploring protein-protein interaction.In addition,we discuss the application of these tools in the investigation of protein-protein interaction networks involved in DNA damage response and DNA repair.  相似文献   

A Mixture IRTree Model for Extreme Response Style: Accounting for Response Process Uncertainty     

Nana Kim  Daniel M. Bolt 《Educational and psychological measurement》2021,81(1):131

This paper presents a mixture item response tree (IRTree) model for extreme response style. Unlike traditional applications of single IRTree models, a mixture approach provides a way of representing the mixture of respondents following different underlying response processes (between individuals), as well as the uncertainty present at the individual level (within an individual). Simulation analyses reveal the potential of the mixture approach in identifying subgroups of respondents exhibiting response behavior reflective of different underlying response processes. Application to real data from the Students Like Learning Mathematics (SLM) scale of Trends in International Mathematics and Science Study (TIMSS) 2015 demonstrates the superior comparative fit of the mixture representation, as well as the consequences of applying the mixture on the estimation of content and response style traits. We argue that methodology applied to investigate response styles should attend to the inherent uncertainty of response style influence due to the likely influence of both response styles and the content trait on the selection of extreme response categories.  相似文献   

Seeing the Forest and the Trees: Comparison of Two IRTree Models to Investigate the Impact of Full Versus Endpoint-Only Response Option Labeling     

Elisabeth M. Spratto  Brian C. Leventhal  Deborah L. Bandalos 《Educational and psychological measurement》2021,81(1):39

In this study, we examined the results and interpretations produced from two different IRTree models—one using paths consisting of only dichotomous decisions, and one using paths consisting of both dichotomous and polytomous decisions. We used data from two versions of an impulsivity measure. In the first version, all the response options had labels; in the second version, only the endpoints were labeled. Based on past research, we hypothesized that the endpoints would be selected more frequently in the endpoint-only labeled condition, and the midpoint response option would be selected more frequently in the fully labeled condition. Results from the two models (dichotomous and polytomous) were similar and indicated that our hypotheses were partially supported—specifically, there was no consistent pattern in terms of which condition saw a higher frequency of midpoint response selection. However, our hypotheses regarding extreme responding in the endpoint-only labeling condition were supported.  相似文献   

Latent Variable Modeling and Adaptive Testing for Experimental Cognitive Psychopathology Research     

Michael L. Thomas  Gregory G. Brown  Virginie M. Patt  John R. Duffy 《Educational and psychological measurement》2021,81(1):155

The adaptation of experimental cognitive tasks into measures that can be used to quantify neurocognitive outcomes in translational studies and clinical trials has become a key component of the strategy to address psychiatric and neurological disorders. Unfortunately, while most experimental cognitive tests have strong theoretical bases, they can have poor psychometric properties, leaving them vulnerable to measurement challenges that undermine their use in applied settings. Item response theory–based computerized adaptive testing has been proposed as a solution but has been limited in experimental and translational research due to its large sample requirements. We present a generalized latent variable model that, when combined with strong parametric assumptions based on mathematical cognitive models, permits the use of adaptive testing without large samples or the need to precalibrate item parameters. The approach is demonstrated using data from a common measure of working memory—the N-back task—collected across a diverse sample of participants. After evaluating dimensionality and model fit, we conducted a simulation study to compare adaptive versus nonadaptive testing. Computerized adaptive testing either made the task 36% more efficient or score estimates 23% more precise, when compared to nonadaptive testing. This proof-of-concept study demonstrates that latent variable modeling and adaptive testing can be used in experimental cognitive testing even with relatively small samples. Adaptive testing has the potential to improve the impact and replicability of findings from translational studies and clinical trials that use experimental cognitive tasks as outcome measures.  相似文献   

Parameter Estimation Accuracy of the Effort-Moderated Item Response Theory Model Under Multiple Assumption Violations     

Joseph A. Rios  James Soland 《Educational and psychological measurement》2021,81(3):569

As low-stakes testing contexts increase, low test-taking effort may serve as a serious validity threat. One common solution to this problem is to identify noneffortful responses and treat them as missing during parameter estimation via the effort-moderated item response theory (EM-IRT) model. Although this model has been shown to outperform traditional IRT models (e.g., two-parameter logistic [2PL]) in parameter estimation under simulated conditions, prior research has failed to examine its performance under violations to the model’s assumptions. Therefore, the objective of this simulation study was to examine item and mean ability parameter recovery when violating the assumptions that noneffortful responding occurs randomly (Assumption 1) and is unrelated to the underlying ability of examinees (Assumption 2). Results demonstrated that, across conditions, the EM-IRT model provided robust item parameter estimates to violations of Assumption 1. However, bias values greater than 0.20 SDs were observed for the EM-IRT model when violating Assumption 2; nonetheless, these values were still lower than the 2PL model. In terms of mean ability estimates, model results indicated equal performance between the EM-IRT and 2PL models across conditions. Across both models, mean ability estimates were found to be biased by more than 0.25 SDs when violating Assumption 2. However, our accompanying empirical study suggested that this biasing occurred under extreme conditions that may not be present in some operational settings. Overall, these results suggest that the EM-IRT model provides superior item and equal mean ability parameter estimates in the presence of model violations under realistic conditions when compared with the 2PL model.  相似文献   

图书馆快速响应服务机制及其实施   总被引：1，自引：0，他引：1  

叶勤 《现代情报》2007,27(11):29-31

快速响应是一种供应链管理方法，因其高效实用在很多行业领域得到应用。本文简述了图书馆快速响应服务的概念，探讨了快速响应服务的特点以及实施的途径与方式。  相似文献   

湖北高等教育摭谈   总被引：1，自引：0，他引：1  

马杰  卢红梅 《湖北广播电视大学学报》2005,22(4):20-22

湖北地处我国中部，是我国的高等教育大省，在全国具有广泛的影响和重要性。在东部跨越式发展和西部大开发的形势下，如何利用承东启西的地理优势促进湖北高等教育的繁荣，最主要的是要结合本区域的特色。本文通过分析湖北高等教育的特点，总结高等教育发展中的经验和发展趋势，旨在能为其他区域高等教育的发展起到一定的借鉴作用。  相似文献   

积极老龄化视域下的老年教育:理念融合与使命创新          下载免费PDF全文

苑大勇刘茹梦 《宁波大学学报(教育科学版)》2023,(3):17-024

当前,老龄化已然成为全球面临的共同挑战,老年教育在应对老龄化社会进程中,应发挥其特有的角色与使命。积极老龄化的理念由世界卫生组织提出,经联合国以及欧盟的不断推进,当前已经形成具有一定实践意义的政策框架与指标体系,得到了国际社会的广泛认同。作为终身学习体系组成部分的老年教育,能够为实现积极老龄化所倡导的“独立健康生活”“就业”以及“社会参与”等政策支柱发挥重要的促进作用。与发达国家相比,我国对老年教育的认知与实践仍有很大的提升空间。在未来,我国应当树立“健康即是回报”的老年教育观念,增加对老年教育的社会支持,通过教育增强老年群体的健康知识学习与社会参与,进而促进积极老龄化的实现。  相似文献   

热膨胀仪稳健设计中的响应曲面方法     

赵小山  吴爱第 《天津工程师范学院学报》2002,12(3):7-10

首先利用了动态特性的响应曲面方法对某热膨胀仪进行了稳健实验设计;然后把得到的优化结果与利用田口的SN比方法得到的优化结果进行了比较,结果表明响应曲面方法较SN比方法有很多优良性质,是对田口玄一博士的SN比方法的一种有效改进途径。  相似文献   

Longitudinal effects of RtI implementation on reading achievement outcomes     

Sally L. Grapin  Nancy Waldron  Diana Joyce‐Beaulieu 《Psychology in the schools》2019,56(2):242-254

Because several studies have investigated student outcomes in schools implementing Response to Intervention (RtI), relatively little research has investigated the impact of implementation on students’ long‐term achievement outcomes (i.e., several years after exposure). The purpose of this study was to describe one elementary school's RtI implementation process and to examine students’ long‐term reading comprehension outcomes following their exposure to various phases of implementation. Four cohorts of students who experienced different implementation phases (i.e., a baseline condition or Phases I, II, or III of implementation) during Grade 2 were subsequently followed across Grades 3, 4, and 5 to examine their outcomes on two reading comprehension measures. Results indicated that students who experienced the early phases of RtI implementation (i.e., Phases I and II) during Grade 2 generally had higher mean comprehension scores in Grades 4 and 5 than students in the baseline condition. Implications for practice and future research are discussed.  相似文献